Krios 5 Cryo-TEM: Cryo-transmission electron microscope for high-fidelity atomic resolution cryo-EM

The 300 kV Krios 5 Cryo-Transmission Electron Microscope (Cryo-TEM) has been designed for high-performance single particle analysis (SPA), cellular cryo-electron tomography (cryo-ET) and microcrystal electron diffraction (MicroED). The Krios 5 Cryo-TEM is capable of quickly producing high-quality structures thanks to a unique focus on high throughput with high data fidelity, increased automation of routine alignments, improved optical precision, and AI-enabled software, which make it a highly productive and “ready-to-use” system for all expertise levels across multiple scientific fields.

Experience up to 25% faster throughput relative to previous Krios Cryo-TEM generations with high data fidelity for single particle analysis. Extended aberration-free image shift (AFIS) accelerates data collection, while AI-powered Smart EPU Software plugins automate routine alignments. Tography 5 Software provides high image quality via automatic 8K Electron Event Representation (EER) decoding and on-the-fly motion correction for crisp tomograms, unlocking the full potential of the Falcon 4i Direct Electron Detector beyond the Nyquist limit. Easily transfer Autogrid cassettes between Autoloaders contamination-free with the Thermo Scientific Vacuum Capsule. Designed for sustainability, the Krios 5 Cryo-TEM features energy-saving Eco modes and environmental footprint traceability with a My Green Lab ACT Ecolabel.

Krios 5 Cryo-TEM benefits for cell and structural biology research

High-data-fidelity atomic-resolution cryo-EM

The Krios 5 Cryo-TEM offers up to 25% faster throughput with high data fidelity, extended aberration-free image shift (AFIS) and magnification calibration accuracy within 1% for single particle analysis. Combined with the optional Thermo Scientific Selectris X Imaging Filter and E-CFEG, the Krios 5 Cryo-TEM can deliver enhanced image contrast for rapid, atomic-resolution imaging.

Faster single particle analysis with AI-automation

AI-assistance with Smart EPU Software enables automated grid screening, quality monitoring, and data management. Smart Plugins in the Smart EPU Software use AI-based image analysis to automate grid area selection and dynamically adjust settings based on real-time analysis.

Accelerated, contamination-free cryo-electron tomography workflow

Tomography 5 Software provides automatic 8K Electron Event Representation (EER) decoding and on-the-fly motion correction for crisp tomograms by unlocking the full data potential beyond the Nyquist limit. Manage tomograms and experimental parameters with the Discovery Viewer in CryoFlow Software.Easily transfer Autogrid cassettes between Autoloaders contamination-free with the Vacuum Capsule.

Applications:

Atomic-resolution single particle analysis

Using a Krios Cryo-TEM equipped with a Selectris X Imaging Filter, structural biologists achieved record-breaking resolution results, featured on the cover of Nature and in other recent articles from Science and Nature. Scientists from the Medical Research Council Laboratory of Molecular Biology in Cambridge, UK, obtained a 1.2 Å resolution structure of the iron-storing protein apoferritin. They also achieved a 1.7 Å resolution for a GABA membrane protein, with even sharper resolution in key parts of the protein. In addition to the leap in resolution, Selectris Filters can also significantly impact productivity; with a better filter and camera, you can achieve a specific resolution with much less data.

Cryo-electron tomography of cells

The Krios 5 Cryo-TEM offers excellent cryo-electron tomography (cryo-ET) capabilities, providing nanometer-scale 3D imaging of a cell’s interior as well as visualization of protein complexes within their physiological environment. Using the Krios Cryo-TEM, tomography data can be obtained from whole bacterial cells or lamellae prepared with quilos, Arctis or Hydra Bio Focused Ion Beam (Cryo-FIB) instruments.