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LumiPoreTM: our photoporation platform
How to use the platform LumiPoreTM nanoparticle transfection workflow
Suitable for R&D and clinical applications l Add nanosensitizers to cell culture medium and leave to incubate (1-30 min) l Add payload of interest to cell culture medium l Perform laser irradiation on LumiPoreTM platform (<10 min. for full 96-well plate) l Optional: wash and add fresh cell medium
LumiPoreTM nanosensitizer-embedded transfection workflow Suitable for cell therapy manufacturing and high-throughput applications
l Either culture cells or add cells in suspension to plate with embedded nanofibers and let cells sediment (1-10 min) l Add payload of interest to cell culture medium l Perform laser irradiation in LumiPoreTM platform (<10 min. for full 96-well plate) l Optional: wash and add fresh cell medium
Advantages of using the LumiPoreTM Platform Excellent cell viability The LumiPoreTM technology is very gentle to cells, ensuring minimal impact on cell viability, cell proliferation, homeostasis and functionality. This has for instance been demonstrated on hard-to-transfect human T cells, which proliferate almost immediately and maintain their full cell-killing potential after transfection with the LumiPoreTM system.
Key: NTC – non-treated cells PEN – photothermal electrospun nanofibers (photoporation) EP – electroporation
Improved yield The LumiPoreTM platform is a gentler technology than the current state-of-the-art electroporation technologies and therefore improves the transfection yield, resulting in a higher number of transfected and viable cells. For instance, photoporation leads to 2.5 times more living mRNA-transfected stimulated human T cells as compared to the current gold standard electroporation method. Reference: Harizaj et al. Adv Funct Mater (2021)
High throughput The LumiPoreTM technology offers high throughput with limited hands-on time, making it suitable for a large variety of applications including screening assays. After adding the proprietary nanosensitizers and effector molecules to the cells, the recipient is placed in the device and the transfection can be started with a simple click of a button. An entire multi-well plate is transfected in less than ten minutes.
Advanced cell targeting LumiPoreTM offers the unique possibility to transfect specific subpopulations of cells. For instance, cells can be transfected according to user-defined spatial patterns. This opens up unique experimental solutions for tissue engineering applications or studying bystander effects.
Illustrated here is the selective delivery of two different fluorescent labels to adjacent neurons, facilitating interaction studies between these cells. Photoporation can deliver different fluorescent labels to adjacent cells with precision targeting.
Flexibility The LumiPoreTM platform offers efficient cytosolic delivery of various payloads, including nucleic acids, proteins and contrast agents. It works efficiently on a broad range of cell types, including hard-to-transfect cells such as stem and immune cells.
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